| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-03-29 |
| タイトル |
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タイトル |
Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
X-ray fluorescence holography |
| キーワード |
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主題Scheme |
Other |
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主題 |
atomic structures |
| キーワード |
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主題Scheme |
Other |
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主題 |
protein structures |
| 資源タイプ |
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資源タイプ |
research report |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Ang, Artoni Kevin R.
Umena, Yasufumi
Sato-Tomita, Ayana
Shibayama, Naoya
Happo, Naohisa
Marumi, Riho
Yamamoto, Yuta
Kimura, Koji
Kawamura, Naomi
Takano, Yu
松下, 智裕
Sasaki, Yuji C.
Shen, Jian-Ren
Hayashi, Kouichi
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurements. This approach was demonstrated by obtaining the Mn Kα hologram pattern from the protein crystal Photosystem II without any X-ray-induced reduction of the Mn clusters. Furthermore, a method to interpret the fluorescence patterns as real-space projections of the atoms surrounding the Mn emitters has been developed, where the surrounding atoms produce large dark dips along the emitter$2013scatterer bond directions. This new technique paves the way for future experiments on protein crystals that aim to clarify the local atomic structures of their functional metal clusters, and for other related XFH experiments such as valence-selective XFH or time-resolved XFH. |
| 書誌情報 |
en : Journal of Synchrotron Radiation
巻 30,
号 2,
p. 368-378,
発行日 2023-01-20
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| 出版者 |
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出版者 |
International Union of Crystallography |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1600-5775 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1107/S1600577522011833 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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識別子タイプ |
URI |
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関連識別子 |
https://journals.iucr.org/s/issues/2023/02/00/rv5166/index.html |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licences/by/4.0/legalcode |
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権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
| 著者版フラグ |
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出版タイプ |
NA |
