| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-03-22 |
| タイトル |
|
|
タイトル |
Microenvironmental Analysis and Control for Local Cells under Confluent Conditions via a Capillary-Based Microfluidic Device |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
| 著者 |
Ota, Nobutoshi
Tanaka, Nobuyuki
Sato, Asako
Shen, Yigang
Yalikun, Yaxiaer
Tanaka, Yo
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Sophisticated functions of biological tissues are supported by small biological units of cells that are localized within a region of 100 μm scale. The cells in these units secrete molecules to form their microenvironment to play a vital role in biological functions. Various microfluidic devices have been developed to analyze the microenvironment but were not designed for cells in a culture dish in a confluent condition, a typical setup for cell and tissue cultivation. This study presents a novel glass capillary-based microfluidic device for studying confluent cells in a culture dish. The multiple capillaries allow the device to confine the local flow in 100 μm or smaller scale to form two adjacent regions with different chemical properties; it can simultaneously perform local cell stimulation and collect secreted molecules from the stimulated cells. Cell removal was achieved upon trypsin stimulation from a limited area (3.8 × 10$20133 ± 1.0 × 10$20133 mm2), which corresponded to 7.6 ± 2.0 cells, using the mouse skeletal myoblast cell line (C2C12 cells) in a confluent condition. Microenvironmental analysis was demonstrated by measuring the secreted tumor necrosis factor alpha (TNF-α) collected from the microenvironment of the stimulated and unstimulated mouse leukemic monocyte cell line (RAW264 cells) to track temporal changes in the TNF-α production. The TNF-α secreted from stimulated cells was approximately four-fold higher than that from unstimulated cells in 90 min. This device enables local cell stimulation and the collection of secreted molecules for cells under confluent conditions, which contributes to the analysis of the cellular microenvironment. |
| 書誌情報 |
en : Analytical Chemistry
巻 94,
号 47,
p. 16299-16307,
発行日 2022-11-16
|
| 出版者 |
|
|
出版者 |
American Chemical Society |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1520-6882 |
| 出版者版DOI |
|
|
関連タイプ |
isReplacedBy |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1021/acs.analchem.2c02815 |
| 出版者版URI |
|
|
関連タイプ |
isReplacedBy |
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://pubs.acs.org/doi/full/10.1021/acs.analchem.2c02815 |
| 権利 |
|
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
$00A9 2022 American Chemical Society. This publication is licensed under CC-BY 4.0. |
| 著者版フラグ |
|
|
出版タイプ |
NA |
