| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-01-18 |
| タイトル |
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タイトル |
Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span |
| 言語 |
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言語 |
eng |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
impedance cytometry |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
electrode gap |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
detection sensitivity |
| キーワード |
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主題Scheme |
Other |
|
主題 |
yeast cells |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Liu, Xun
Tang, Tao
Yi, Po-Wei
Yuan, Yapeng
Lei, Cheng
Li, Ming
Tanaka, Yo
細川, 陽一郎
Yalikun, Yaxiaer
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Impedance cytometry is wildly used in single-cell detection, and its sensitivity is essential for determining the status of single cells. In this work, we focus on the effect of electrode gap on detection sensitivity. Through comparing the electrode span of 1 $00B5m and 5 $00B5m, our work shows that narrowing the electrode span could greatly improve detection sensitivity. The mechanism underlying the sensitivity improvement was analyzed via numerical simulation. The small electrode gap (1 $00B5m) allows the electric field to concentrate near the detection area, resulting in a high sensitivity for tiny particles. This finding is also verified with the mixture suspension of 1 $00B5m and 3 $00B5m polystyrene beads. As a result, the electrodes with 1 $00B5m gap can detect more 1 $00B5m beads in the suspension than electrodes with 5 $00B5m gap. Additionally, for single yeast cells analysis, it is found that impedance cytometry with 1 $00B5m electrodes gap can easily distinguish budding yeast cells, which cannot be realized by the impedance cytometry with 5 $00B5m electrodes gap. All experimental results support that narrowing the electrode gap is necessary for tiny particle detection, which is an important step in the development of submicron and nanoscale impedance cytometry. |
| 書誌情報 |
en : Sensors
巻 22,
号 20,
発行日 2022-10-12
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| 出版者 |
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出版者 |
MDPI |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1424-8220 |
| 出版者版DOI |
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関連タイプ |
isReplacedBy |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3390/s22207743 |
| 出版者版URI |
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関連タイプ |
isReplacedBy |
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|
識別子タイプ |
URI |
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関連識別子 |
https://www.mdpi.com/1424-8220/22/20/7743 |
| 権利 |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
$00A9 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| 著者版フラグ |
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|
出版タイプ |
NA |
